Figure 1

Study Schedule. Plasma content of PEGylated MNPs and p-PEGylated MNPs 5 min, 1 h, 3 h, 6 h, 24 h, 48 h, 7 days, 21 days, 28 days, 42 days and 56 days post-administration, and tissues, includes, heart, lungs, brain, liver, kidneys, spleen, thymus, testes, and epididymides were collected on 14 days and 56 days post-administration. Clinical symptoms were observed every day, while animal body mass and food intake were measured every week. Hematology and CD4⁺/CD8⁺ lymphocyte determination were performed on 7,14,28 and 56 days post-administration, the biochemical detection were performed on 14 and 56 days post-administration, and the cytokine profiles of animals were detected on 0, 7,14 days, and 56 days post-administration. Animals were anesthetized on 14 days and 56 days post-administration respectively for necropsy and histopathological examination. Comet assay was performed on 3 h, 24 h, 48 h and 14 days post-administration for the peripheral blood, and 14 days and 56 days post-administraion, respectively. Pig-a gene mutation for RBC^CD59- were performed before the administration, and on 14, 28, 42 and 56 days post-administration, respectively.