Figure 5 | Scientific Reports

Figure 5

From: Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1

Figure 5

A Virotrap-based assay identifies calpain small subunit 1 (CAPNS1) as a possible TGM1 interactor. (a) Virotrap principle. A protein consisting of HIV-1 p55 Gag protein fused to the bait (TGM1, eDHFR as a negative control) is transiently expressed in HEK293T cells. Gag directs this fusion protein to the cell membrane and induces budding of virus-like particles (VLPs) in which interaction partners of the bait protein are trapped. The released VLPs are purified and the proteins are identified by mass spectrometry. (b) Volcano plot showing TGM1 interactors identified by Virotrap-based screening. TGM1-specific interaction partners are shown on the left side of the volcano plot and eDHFR-specific interaction partners are shown on the right side of the volcano plot. (c) The scheme depicts CAPNS1 interactions with calpain 1 (CAPN1) and calpain 2 (CAPN2) through the fifth penta-EF-hand (PEF) domain. eDHFR, Escherichia coli dihydrofolate reductase; CysPC1/2, cysteine protease core domain 1/2; CBSW, calpain-type β-sandwich.

Back to article page