Figure 8

Protease pretreatment of skin sections facilitates immunolabeling of TGM1 in cornified cells. Human toe skin was immuolabeled for TGM1 without (a, d) or with pretreatment of the section with proteinase K (b) or trypsin (e). In negative control experiments (e, f), the sections were pretreated and the anti-TGM1 antibody was replaced by immunoglobulin G from non-immunized animals. epi, epidermis; sc, stratum corneum.