Table 1 Primer sequence and annealing temperature.
From: Significance of LHCGR polymorphisms in polycystic ovary syndrome: an association study
Polymorphism | Primer set | 5′–3′ sequence | Annealing temp |
|---|---|---|---|
rs4953616 | Forward inner | ATCCTCATCATCATTTCCATTATCCC | 65.5 °C |
Reverse inner | TGAGGGTTAGGCTTCTTGGCA | ||
Forward outer | TCCATATAGTTTGCTGAAGGAGGGA | ||
Reverse outer | TTTCAACACCCAGAAAACCCATATG | ||
rs7371084 | Forward | GCCAGGCACAGCGTTAAG | 63 °C |
Reverse | GCTGTCAGTCTGAAGTTGGG |
