Figure 10

Representative CLSM highlighting the differences in growth of E.coli (green fluorescence) on the 20% fat tri-phasic 3D viscoelastic model surface at all growth stages, i.e., lag phase (3 h), mid- (5 h) and late-exponential (7 h), as well as at (late) stationary phase (24 h). Colonies located in fat rich environments are annotated with circles and colonies located on a fat-poor environment are annotated with squares. Fat is stained with NileRed (red fluorescence).