Table 2 Characteristics of markers.
Locus | Position on chr. X (bp) | Primer sequence (5’–3’) and 5’ label | Tm | Conc [µM] | Motif | Allele (bp) |
|---|---|---|---|---|---|---|
Multiplex A | ||||||
Ld4901 | 56,217,757 56,217,907 | f: PET-ACACAATCATTCTAGCGCCG r: TTGGAAGCATAATCGTGGCAG | 57.3 63.7 | 0.08 0.08 | (TAA)7 | 144–147 |
Ld4903 | 56,256,738 56,256,948 | f: 6-FAM-ACTGTAGAATCGCGGCTATGA r: TCATTGTTTTATGTTTCCTGCGT | 57.9 61.3 | 0.04 0.04 | (AAC)5 | 214 |
Ld4905 | 56,323,276 56,323,575 | f: VIC-GCGTCTTAGATGTCAATGCCT r: CATATCTGATCGAGGAAGGTGT | 57.9 63.9 | 0.04 0.04 | (TTCA)5 | 295–300 |
Ld4908 | 56,350,983 56,351,137 | f: 6-FAM-GCACGAACTCTTCAAACGGT r: TGCCATTTCGCCCTTTCAT | 57.3 61.6 | 0.04 0.04 | (AAT)5 | 155 |
Ld6111 | 52,920,157 52,920,335 | f: NED-AAAGTTGAACGTGATGGTTGG r: TCCGTTGGCTTTGAAACCT | 55.9 61.6 | 0.08 0.08 | (TAT)5 | 174 |
Multiplex B | ||||||
Ld6102 | 52,524,306 52,524,557 | f: 6-FAM-TCACACCAATAGCTGCAATGA r: CAGGAGGATAGCACCAGTGA | 55.9 65.0 | 0.08 0.08 | (ACTC)8 | 244–252 |
Ld6105 | 52,598,946 52,599,155 | f: PET-CCTGCATCGATTCCTTGTG r: CCACACAAACCTCTGACATATCT | 56.7 64.0 | 0.08 0.08 | (TGG)7 | 208–211 |
Ld6106 | 52,659,499 52,659,746 | f: NED-CCCCTTTTCCGCTAATCTTC r: ACCGGTGGATGACAATATTCA | 57.3 62.2 | 0.08 0.08 | (ATT)6 | 236–242 |
Ld6109 | 52,841,444 52,841,758 | f: VIC-TTTTAACACAGCCGGACGAT r: GGCTCCCACAAGAAATACAGA | 55.3 63.7 | 0.08 0.08 | (CTTAC)5 | 309–319 |
Ld6110 | 52,845,079 52,845,314 | f: VIC-GAAGCGCAATTGAATGATGAC r: TTTGGAGCCACTTCTGTTTACT | 55.9 62.4 | 0.04 0.04 | (TAT)5 | 232 |
