Table 1 Effects of MCFA + NT on in vitro gas and methane production, apparent dry matter disappearance (aDMd), ammonia-N concentration, and pH.

From: Combined effects of nitrate and medium-chain fatty acids on methane production, rumen fermentation, and rumen bacterial populations in vitro

Treatment

TGP† (mL/g DMi§)

Methane

aDMd‡ (g/g)

Net NH3–N (mg/100 mL)

pH

(mL/g DMi)

(% TGP)

(mL/g aDMd)

(mol/mol nVFA)

Control

298.7

36.5

12.22

57.6

0.2125

0.6345

13.1

5.90

NT

289.3*

30.2*

10.42*

48.1*

0.1746

0.6313

20.7*

6.06*

NT + C8

286.6*

27.7*

9.64*

42.4*

0.1599*

0.6539

20.9*

6.06*

NT + C10

263.8*

21.9*

8.29*

34.6*

0.1498*

0.6372

24.7*

6.27*

NT + C12

282.8*

27.9*

9.84*

43.7*

0.1592*

0.6405

21.0*

6.13*

NT + C14

291.7

30.7*

10.51*

51.9*

0.1727

0.5923*

21.3*

6.08*

NT + C8/C10

277.9*

25.7*

9.23*

39.3*

0.1825

0.6569

24.0*

6.18*

NT + C8/C12

284.0*

26.9*

9.48*

41.2*

0.1678*

0.6565

20.9*

6.13*

NT + C8/C14

288.8*

29.1*

10.06*

45.6*

0.1637*

0.6402

22.4*

6.05*

NT + C10/C12

271.0*

24.0*

8.88*

38.4*

0.1478*

0.6315

22.7*

6.20*

NT + C10/C14

278.8*

26.1*

9.36*

41.0*

0.1511*

0.6383

21.2*

6.15*

NT + C12/C14

283.1*

27.9*

9.85*

45.2*

0.1548*

0.6184

21.9*

6.09*

SEM

2.53

0.65

0.163

1.22

0.00484

0.00517

0.93

0.020

P-value

< 0.001

< 0.001

< 0.001

< 0.001

0.009

0.002

< 0.001

< 0.001

  1. †Total gas production; ‡apparent dry matter disappearance; §dry matter incubated; ¶net production of volatile fatty acids.
  2. *Means within a column differ significantly (P < 0.05) from the corresponding control (0 mg/L). SEM, standard error of the mean.
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