Table 4 Comparison of parameters determined in the present work with previous studies.

From: Molecular characterization of a galactomannan extracted from Tara (Caesalpinia spinosa) seeds

Parameter

Value

Method

References

Man/Gal ratio

3.37

HPAEC—PAD

Present work

 

4.2

FT-IR and GC–MS

40

2.9

HPAEC

48

1.54–1.85

NMR

29

3

HPAEC

30

Protein content

0.74%

Dumas

Present work

 

 < 3.5% (FAO/WHO)

49

0.71%

NA2100 Nitrogen and Protein Analyzer

30

Specific refractive index (dn/dc)

0.1454

Refractive index detector from AF4 equipment

Present work

Molecular weight

(2.46–3.69) × 107 Da

AF4 (average of distribution)

Present work

 

9.14 × 103 KDa

Dynamic Light Scattering (DLS)

Present work

1519 × 103 Da

Gel permeation

50

2.18 × 106 Da

Static Light Scattering(SLS)

51

2.23 × 106 Da

As viscosity average molecular weight

30

1300–1500 KDa

Calculated from intrinsic viscosity

29

3.7 × 106 Da

Calculated from intrinsic viscosity

52

Radius of gyration

(231.1–281.7) nm

AF4 (average of distribution)

Present work

 

(73–80) nm

Calculated from intrinsic viscosity

29

Hydrodynamic radius

(43.804–45.052) nm

AF4

Present work

 

(77–85) nm

Calculated from hydrated volume

29

63 nm

Calculated from intrinsic viscosity

52