Table 4 Comparison of parameters determined in the present work with previous studies.
From: Molecular characterization of a galactomannan extracted from Tara (Caesalpinia spinosa) seeds
Parameter | Value | Method | References |
|---|---|---|---|
Man/Gal ratio | 3.37 | HPAEC—PAD | Present work |
4.2 | FT-IR and GC–MS | ||
2.9 | HPAEC | ||
1.54–1.85 | NMR | ||
3 | HPAEC | ||
Protein content | 0.74% | Dumas | Present work |
< 3.5% (FAO/WHO) | – | ||
0.71% | NA2100 Nitrogen and Protein Analyzer | ||
Specific refractive index (dn/dc) | 0.1454 | Refractive index detector from AF4 equipment | Present work |
Molecular weight | (2.46–3.69) × 107 Da | AF4 (average of distribution) | Present work |
9.14 × 103 KDa | Dynamic Light Scattering (DLS) | Present work | |
1519 × 103 Da | Gel permeation | ||
2.18 × 106 Da | Static Light Scattering(SLS) | ||
2.23 × 106 Da | As viscosity average molecular weight | ||
1300–1500 KDa | Calculated from intrinsic viscosity | ||
3.7 × 106 Da | Calculated from intrinsic viscosity | ||
Radius of gyration | (231.1–281.7) nm | AF4 (average of distribution) | Present work |
(73–80) nm | Calculated from intrinsic viscosity | ||
Hydrodynamic radius | (43.804–45.052) nm | AF4 | Present work |
(77–85) nm | Calculated from hydrated volume | ||
63 nm | Calculated from intrinsic viscosity |