Figure 2
G⍺12 knockdown does not alter mouse survival or tumor size observed following orthotopic intracranial injection of GSC tumor cells. shRNA control or shG⍺12 knockdown GSC23 and HK281 cells labeled with IRFP720 were intracranially injected into syngeneic nu/nu mice. (A, B) Kaplan–Meier survival curves for GSC23 and HK281 control and shG⍺12 KD tumor-bearing mice (6 animals per group, in 2 independent experiments for GSC23, and 4 animals per group for HK281 in one experiment). Survival curves were not significantly different as determined using the Log rank test (C, D) Brain tumor growth of GSC23-engrafted mice as monitored using Fluorescence Molecular Tomography (FMT) emission at 720 nm. Representative FMT scan images at 17 days post intracranial injection and relative fluorescence quantification. (E) Additional set of animals injected with approximately 3 × 105 GSC23 cells and analyzed for tumor growth monitored by FMT did not show significant differences in tumor size (p = 0.1; n = 6).
