Figure 5 | Scientific Reports

Figure 5

From: Gα12 signaling regulates transcriptional and phenotypic responses that promote glioblastoma tumor invasion

Figure 5

G⍺12 knockdown GSCs tumors have altered gene expression profiles compatible with phenotypic changes in GSC cell behavior observed in vitro. (A) mRNA expression of proneural-mesenchymal transition related genes in GSC23 tumor samples (n = 4, *p < 0.05, **p < 0.01 vs. shControl). (B) Cell plasticity of GSC23 grown on Matrigel-coated surfaces for 21 days visualized by light microscopy (20X). (C) mRNA expression of cancer-associated stem cell genes in GSC23 tumor samples (n = 8, *p < 0.05 vs. shControl). The total number of GSC23 spheres formed at 14 days in culture plotted by extreme limiting dilution analysis (ELDA, 0.95 confidence interval). Estimated stem cell frequency per group in the table.

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