Figure 2 | Scientific Reports

Figure 2

From: Human iPSC-derived microglia carrying the LRRK2-G2019S mutation show a Parkinson’s disease related transcriptional profile and function

Figure 2

Mitochondrial respiration in LRRK2-G2019S carrying iMGL. Mitochondrial respiration of iMGL was measured with Seahorse Mito Stress test. (a) Schematic for the assay. Oxygen consumption rate (OCR) of iMGL was measured in response to sequential injections of oligomycin (Olig), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and a combination of rotenone and antimycin A (R + A) (b) in female isogenic iMGL pair and (c) in male iMGL pair and–CTRL and m-PD at the basal level and after IFNγ stimulation. (d,e) Mitochondrial parameters calculated from changes in the OCR. n = 3 batches (f-ISO1 and f-PD1) and n = 4 batches (m-CTRL and m-PD), with at least 7 technical replicates (wells) per group in both. Data are presented as mean ± SD. Two-way Anova with Sidak´s multiple comparison test, *p < 0.05, **p < 0.01. Any wells with negative OCR readings were considered outliers and excluded from the analysis.

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