Table 2 The cycle program followed these steps.
From: Evaluation of antibacterial properties of nisin peptide expressed in carrots
nptII (Ċ/min) | Nisin (Ċ/min) | Vir gen (Ċ/min) | |
|---|---|---|---|
Initial denaturation | 94/2 min | 94/2 min | 94/5 min |
Denaturation | 94/1 min | 94/1 min | 94/1 min |
Annealing | 55/1 min | 62/1 min | 58/1 min |
Extension | 72/3 min | 72/1 min | 72/1 min |
Final extension | 72/5 min | 72/5 min | 72/7 min |
Cycle | 35 | 35 | 35 |
