Figure 3

Oligodendroglial cell densities and proliferation are decreased in Sox8^−/− mice. Mice received a 0.2% cuprizone diet for 5 weeks and were fed normal chow for 7 additional days. Immunohistochemical staining of the CC for (A) NogoA⁺ and (B) Olig2⁺ oligodendroglial cells in Sox8^−/− mice or WT controls was performed at full demyelination (wk 5; n = 6–7 per group) and during remyelination (wk 5.5; n = 9 per group and wk 6; n = 4 per group) compared to naïve mice without cuprizone feeding (n = 4–5 per group). Representative pictures of the median body of the CC are shown for each staining and group. Scale bar is 200 µm. (C) Immunofluorescence staining for Olig2 (green) and Ki67 (red) revealed reduced proliferation of Olig2⁺ cells in Sox8^−/− mice at wk 5 (n = 4–7 per group and time point). Exemplary images of Ki67⁺Olig2⁺ cells are shown. Bars represent mean, error bars show SEM.