Figure 4
Astrogliosis and microglia activation after toxic demyelination. Mice received a 0.2% cuprizone diet for 5 weeks and were fed normal chow for 7 additional days. Histochemical staining of the CC with (A) anti-GFAP and (B) anti-Iba1 was performed at full demyelination (wk 5; n = 6–7 per group) and remyelination (wk 5.5; n = 7–9 per group and wk 6; n = 4 per group) in Sox8−/− mice or WT controls compared to naïve mice without cuprizone feeding (n = 4–5 per group). Representative pictures of the median body of the CC are shown for each staining and group. Scale bar is 200 µm. Bars represent mean, error bars show SEM.
