Figure 2
From: Human cell-expressed tag-free rhMFG-E8 as an effective radiation mitigator
(a) Tag-free rhMFG-E8 is superior to E coli expressed His-tagged rhMFG-E8. PS binding (left panel) and SVEC4-10 cells adhesion assay (right panel) using tag-free and His-tagged rhMFG-E8. (b) Tag-free rhMFG-E8 has high biological activity. PS binding (left panel) and SVEC4-10 cell adhesion assay (right panel) of commercial MFG-E8 (R&D MFG-E8) and tag-free rhMFG-E8 (rhMFG-E8). (c,d) Tag-free rhMFG-E8 is highly stable. PS binding and cell adhesion assays performed at (c) twelve months, (d) twenty-four months after storage of the tag-free rhMFG-E8 at − 20 °C.
