Figure 1

Two-way cluster analysis of bacterial DNA (bactDNA) from T-RFLP profiles performed in duodenal fluid samples. MspI was used as restriction endonuclease and Bray–Curtis index (deviation = 3) served for comparison. Duodenal samples (n = 125) are represented by rows, according to the study group color code on the right side of the graph (black: cirrhosis; grey: controls). A second legend presents the subgroup of duodenal samples that were analyzed by amplicon deep sequencing. It includes those samples whose paired blood was bactDNA-positive (orange; n = 7), whose paired ascites was bactDNA-positive (blue; n = 7), selective samples with bactDNA negative blood and ascites (green; n = 15) and controls (grey; n = 11). Duodenal samples are clustered according to the similarity of their T-RFLP profile. T-RF in base pairs are represented by columns and clustered by co-occurring T-RF within the sample. The color key (upper right corner) shows the relative abundance of the T-RF.