Figure 3

Expression of apoptosis-related genes (p21 and p53), cell cycle-related genes (cyclin B1 and PCNA1), MDR1, and Drp1. RNAs were isolated form untreated or treated cells, reverse transcribed, and then used as templates in qRT-PCR reactions. In PTX-resistant cells, the expression of p53 and p21 increased (A), meanwhile cyclin B1 and PCNA1 were downregulated (B) relative to the untreated resistant cells. MDR1 expression was higher in resistant cells compared to sensitive cells (Control-S), and decreased in dually-treated resistant cells compared to the corresponding untreated cells. No significant difference was seen between normal and resistant cells; however it decreased in dully treated resistant cells.