Figure 5 | Scientific Reports

Figure 5

From: Beneficial effects of bio-fabricated selenium nanoparticles as seed nanopriming agent on seed germination in rice (Oryza sativa L.)

Figure 5

Effect of different concentrations of SeNPsnanopriming at different intervals on (a) superoxide generation using NBT staining (b) hydrogen peroxide generation using DAB staining (c) Peroxidase activity was assessed by staining with TMB in rice seeds imbibed for 24 and 48 h and (e) for 72 h respectively (d) seedling vigor by TTC staining in rice seeds imbibed for 18 and 24 h and (f) for 72 h respectively. (Scale bar = 2 mm). (g) Seedlings after 10 days of germination (Scale bar = 10 cm). (h) Seedlings after 3 days of germination (Scale bar = 2 cm). (i) TTC staining of horizontal cut section of rice seed (Scale bar = 5 mm). (T1 (Control), T2 (Se 10), T3 (Se 20), T4 (SeNP 20 and T5 (SeNP 25).

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