Figure 3

Overexpression of miR-205-5p promotes cell proliferation and migration of KKU-100 cells in vitro. (a) Relative expression of miR-205-5p to miR-16 as the internal control in the KKU-100 CCA cell line at 48 h after transfection with an miR-205-5p mimic. (b) MTT assay to measure relative proliferation rates of KKU-100 cells transfected with miR-205-5p mimic compared to the mock-transfected controls at all time points (24, 48, and 72 h). (c, d) The relative cell migration rates of KKU-100 cells transfected with miR-205-5p mimic compared to mock-transfected controls at 12, 24, 48, and 72 h. Experiments were performed in triplicate and expressed as mean ± SD (*p < 0.05).