Table 1 Primers sequences used for gene amplification with qPCR and/or ddPCR.
Gene | Primer sequence (5′–3′) | Accession number |
|---|---|---|
GAPDH (reference gene) | F: TGCACCACCAACTGCTTAGC R: GGCATGGACTGTGGTCATGAG | NM_002046.5 |
RPLO (reference gene) | F: TGCAGCTGATCAAGACTGGAGACA R: TCCAGGAAGCGAGAATGCAGAGTT | BC_001834.2 |
ACTB3 | F: GGCCGAGGACTTTGATTGCAC R: TTAGGATGGCAAGGGACTTCCTGT | NM_001101.3 |
AKT1 | F: TGTCATCGAACGCACCTTCC R: ACACCTCCATCTCTTCAGCC | NM_005163.2 |
TGFβ-1 | F: CTCGCCAGAGTGGTTATCTT R: AGTGTGTTATCCCTGCTGTC | NM_000660.7 |
Vimentin | F: CCT CTT CCA AAC TTT TCC TCC R: CGT TGA TAA CCT GTC CAT CTC | NM_003380.5 |
ESR1 | F: TATGTGTCCAGCCACCAACC R: TCGGTCTTTTCGTATCCCACC | NG_011535.1 |
ESR2 | F: CGGCAGACCACAAGCCCAAATG R: CGATCTTGCTTCACACCAGGGAC | NG_008493.2 |
