Figure 4
From: Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1 receptor genes
Feeding A. aegypti larvae with 5-HTR.426 yeast causes neural defects. Larval brains were labeled with three antibodies: mAbnc82 (an active synapse marker; white in (a1, a2); green in (c1, c2)), anti-HRP (neural marker; white in (b1, b2); red in (c1, c2)), and TO-PRO (nuclear marker; blue in (c1, c2)). The levels of nc82, a protein involved in neural development, were significantly reduced in the synaptic neuropil of larvae fed with 5-HTR.426 yeast (a2, c2) compared to control yeast-treated (control) larvae (a1, c1). The data are presented as average mean gray values, with error bars denoting standard error of the mean. ***Indicates a statistically significant difference from the control group (P < 0.001). Representative larval brains are oriented dorsal upward in this figure. LAL, larval antennal lobe; OL, optic lobe; SOG, sub-esophageal ganglion; SuEG, supraesophageal ganglion. Scale Bar = 100 μm.
