Figure 1 | Scientific Reports

Figure 1

From: A simple and rapid assay of lysosomal-targeting CDy6 for long-term real-time viability assessments in 2D and 3D in vitro culture models

Figure 1

CLV-DMSO extraction from CDy6-stained HaCaT cells can measure intercellular lysosomal vesicle levels at an absorbance spectrum of 570 nm. (A,B) Fluorescence live-cell imaging of CDy6-labeled HaCaT cells after CDy6 staining (100 ng/mL) for 1 h. The specificity and patterns of the staining very visibly display the form of the lysosomal vesicles in the cytoplasm. Scale bars, 200 µm (A) and 20 µm (B). (C,D) The absorbance spectra of the DMSO only and the CLV-DMSO extract from CDy6-stained HaCaT cells were measured with an ELISA reader. DMSO is used as a negative control. The red arrow indicates the peak excitation/emission wavelengths of CDy6, reaching 570/585 nm.

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