Figure 2 | Scientific Reports

Figure 2

From: A simple and rapid assay of lysosomal-targeting CDy6 for long-term real-time viability assessments in 2D and 3D in vitro culture models

Figure 2

Comparative assessment between CDy6-stained and calcein AM-stained HaCaT cells before and after DMSO extraction. (A,B) After staining with calcein AM (100 nM) and CDy6 (100 ng/mL), fluorescence images of live cells were obtained using a confocal microscope at the indicated time points as described in more detail in the materials and methods section. (C,D) The absorbance spectrum of the CLV-DMSO and calcein AM-DMSO extracts from each group was measured with an ELISA reader. The red arrow indicates the peak excitation/emission wavelengths of CDy6, reaching 570/585 nm.

Back to article page