Figure 3 | Scientific Reports

Figure 3

From: A simple and rapid assay of lysosomal-targeting CDy6 for long-term real-time viability assessments in 2D and 3D in vitro culture models

Figure 3

Comparative analysis of CDy6 and calcein AM staining in HaCaT cells before and after washing. (A,B) Live cell fluorescence images were obtained using a confocal microscope at specified time points after staining HaCaT cells with CDy6 (100 ng/mL) and calcein AM (100 nM) with and without washing, as described in detail in the Materials and methods section as well as in Fig. S2 in the Supplementary information. (C–F) Absorbance spectra of the CLV-DMSO and calcein AM-DMSO extracts were measured with an ELISA reader before and after washing each sample. The red arrow indicates the peak excitation/emission wavelengths of CDy6, reaching 570/585 nm. Group 1 includes samples that underwent 7 days of cultivation in the presence of each dye. Group 2 includes samples stained with each dye for 1 h, followed by washing, and 7 days of cultivation.

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