Figure 4 | Scientific Reports

Figure 4

From: A simple and rapid assay of lysosomal-targeting CDy6 for long-term real-time viability assessments in 2D and 3D in vitro culture models

Figure 4

Effect of CDy6 and Calcein AM staining on cell viability in HaCaT cells under the long-term culture condition. (A) HaCaT cells were stained with CDy6 (100 ng/mL) or Calcein AM (100 nM) for 7 days. Cell viability was assessed by crystal violet staining. Representative images of crystal violet staining after 7 days are shown. (B) Bar graph showing cell proliferation in each group: untreated (pink), calcein AM (blue), and CDy6 (green). Data represent the mean ± SD from three independent experiments performed in triplicate as a percentage of the untreated group. Data was analyzed using Student’s t-test. ***P < 0.001 versus corresponding controls.

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