Figure 7 | Scientific Reports

Figure 7

From: A simple and rapid assay of lysosomal-targeting CDy6 for long-term real-time viability assessments in 2D and 3D in vitro culture models

Figure 7

Analysis of the CDy6-stained 3D dermal layer using CLVs and Western blot. (A) CLV-DMSO extracts were obtained from CDy6-stained 3D dermal layers at the indicated time points, and the living cells were quantified using an ELISA reader. Representative images of the CLV-DMSO extracts are shown. Data represent the mean ± SD from three independent experiments performed in triplicate as a percentage of the untreated group. Data was analyzed using Student’s t-test. ***P < 0.001 versus corresponding controls. (B) Western blot analysis of caspase expression in each group, with actin as a loading control.

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