Figure 1 | Scientific Reports

Figure 1

From: Renoprotective effects of extracellular fibroblast specific protein 1 via nuclear factor erythroid 2-related factor-mediated antioxidant activity

Figure 1The alternative text for this image may have been generated using AI.

FSP1 enhances the expression of factors associated with Nrf2 activation in TECs in a RAGE-dependent manner. (a) A microarray result. Red dots are genes whose expression levels are more than double by the administration of FSP1 (FSP1+), and green dots are genes whose expression levels were less than half by the administration of FSP1. (b) Quantitative real-time PCR analysis of mRNA showed that heme oxygenase 1 (HO-1), solute carrier family 7, member 11 (Slc7a11), cystathionine gamma-lyase (CSE), and sequestosome 1 (sqstm 1) were all up-regulated in mProx cells treated with FSP1. These effects were blocked after RAGE knockout (RAGE−/−) in mProx cells (n = 5 in each group). (c) Western blots showing AKT and pAKT levels in wild-type and RAGE−/− mProx cells treated with or without FSP1. (d) Densitometric analysis of the blots. FSP1 significantly increased phosphorylated AKT levels (P = 0.029) in a RAGE-dependent manner (n = 4 in each group).

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