Figure 5
Time kill study. Time dependent kill analysis was performed by treatment of early log-phase cells of E. coli U1007 strain with A) monophages (U1G, CR and M) and B) phage combinations (U1G + CR, U1G + M, U1G + CR + M)- with and without colistin) and the samples from each group were retrieved at specific time points from 0– 24 h, serially diluted and plated on to LA plates and incubated at 37 °C. The colony count was expressed as log (CFU/ml). The experiment was performed in triplicates and the error bar represents their standard error of the mean. Student t test shows that triple phage combination with colistin was statistically significant relative to untreated control with a P value of 0.0039, similarly triphage along with colistin displayed significant variation relative to triphage cocktail without colistin (P = 0.052).
