Figure 4
From: S9.6-based hybrid capture immunoassay for pathogen detection
HC-LFA can detect simulated B. anthracis infections in mice. (A) Schematic of HC-LFA. S9.6-GNP = gold nanoparticle-conjugated S9.6. The arrow indicates the direction of mobile phase. Illustration created with BioRender.com. (B) Images of lateral flow assays showing HC-LFA’s specificity for hybrids by probe type (B. anthracis RNA, 2 µg). (C) Images of lateral flow assays showing HC-LFA to detect rpoB transcripts in cellular RNA from B. anthracis or B. anthracis-spiked murine spleen tissue. The spiked bacterial load ranged from 107 to 109 CFU/100 mg of tissue. For bacteria alone, 2 µg cellular RNA, equivalent to a bacterial load of 109 CFU was diluted. 5 ng of P1 probe was used in hybridization and ssDNA controls. Assays were imaged after 30 min. Individual lateral flow strips are placed separately and are grouped within the dashed line in (B) and (C).
