Figure 5
From: S9.6-based hybrid capture immunoassay for pathogen detection
HC assays can detect RNA transcripts from T. gondii and SARS-CoV-2. (A) Average fold change in chemiluminescence from HC-ELISA to detect B1 gene mRNA of T. gondii-infected HFF cells over uninfected cells seeded at the same density at start of study and harvested at the same time as infected cells. The P10 probe was used in this experiment and C (control) is the probe hybridized with cellular RNA from overly confluent uninfected cells. Bars represent mean ± SD of duplicate wells in a representative experiment of n = 3. (B) Median chemiluminescence from HC-ELISA to detect in vitro synthesized Spike E mRNA of SARS-CoV-2. Data obtained using 2 × 109 molecules/well of specified nucleic acids (assuming full hybridization from equal amounts RNA and probe for P10 hybrid). Bars represent range of triplicate wells in a representative experiment of n = 3. (C) Lateral flow assays show the sensitivity of HC-LFA to Spike E gene hybrids generated from 5 ng (2.6 × 109 molecules) P1 probe and in vitro-synthesized mRNA in amounts specified. Assays were imaged after 30 min. Individual lateral flow strips are placed separately and are grouped within the dashed line.
