Table 2 Primers for RT-PCR.
From: AMPKα2 regulates fasting-induced hyperketonemia by suppressing SCOT ubiquitination and degradation
Primer name | DNA sequence 5′–3′ |
|---|---|
Mouse GAPDH-F | ATTGTCAGCAATGCATCCTG |
Mouse GAPDH-R | ATGGACTGTGGTCATGAGCC |
Human GAPDH-F | GGAGCGAGATCCCTCCAAAAT |
Human GAPDH-R | GGCTGTTGTCATACTTCTCATGG |
Human AMPKα2-F | GACTTCCTTCACAGCCTCATC |
Human AMPKα2-R | CGAGCGACTATCAAAGACATACG |
Mouse SCOT-F | CATAAGGGGTGTGTCTGCTACT |
Mouse SCOT-R | GCAAGGTTGCACCATTAGGAAT |
Human SCOT-F | GGGTCCATATCCACGACAACA |
Human SCOT-R | GACGTGTCCACCTCTAATCATTG |
Mouse ACAT1-F | CAGG-AAGTAAGATGCCTGGAAC |
Mouse ACAT1-R | TTCACCCCCTTGGATGACATT |
Human ACAT1-F | ATGCCAGTACACTGAATGATGG |
human ACAT1-R | GATGC-AGCATATACAGGAGCAA |
Mouse BDH1-F | TTCCCCTTCTCCGAAGAGC |
Mouse BDH1-R | CCCAGAGGGTGCATCTCATAG |
Human BDH1-F | GACAGC-CTAAACAGTGACCGA |
Human BDH1-R | GAGCGGACAATCTCCACCA |
