Figure 4
Results of motility measurements. Bar charts show outcome of motility studies, including the measurement of dry- (A) and wet (B) fecal pellet weight in Control-vehicle-treated, Control-clodronate-treated, DSS-vehicle-treated and DSS-clodronate-treated mice. The weight of dry fecal pellets collected in 3 h was significantly higher in the DSS-vehicle group (n = 11) compared to the Control-clodronate (n = 14, p = 0.016) and DSS-clodronate groups (n = 12, p = 0.032). There was no significant difference between the Control-clodronate and DSS-clodronate groups (p = 0.363) and between the Control-vehicle and the Control-clodronate groups (p = 0.74) (A). Regarding wet fecal pellet weight, the same tendencies occurred, with significant differences between the DSS-vehicle and the Control-clodronate groups (p < 0.001) and the DSS-vehicle and DSS-clodronate groups (p = 0.007). Wet fecal pellet output of DSS-clodronate-treated mice was similar to Control-clodronate mice, with no significant difference (p = 0.114), just like in the case of Control-vehicle and Control-clodronate groups (p = 0.829) (B). When applying Evans blue through oral gavage, DSS-vehicle-treated animals (n = 6) showed significantly decreased transit times compared to both Control-clodronate (n = 8, p = 0.001) and DSS-clodronate-treated littermates (n = 6, p = 0.004). There were no significant differences detected between neither the Control-clodronate and DSS-clodronate groups (p = 0.249), nor the Control-vehicle and Control clodronate groups (p = 0.212). Metric data are shown as mean and corresponding standard deviation (SD). Statistical significance *p < 0.05; **p < 0.01, ***p < 0.001.
