Figure 6 | Scientific Reports

Figure 6

From: Depletion of muscularis macrophages ameliorates inflammation-driven dysmotility in murine colitis model

Figure 6

Tissue expression of Atf4, Bax1, Egr1, IL-18 and Hif1a proteins. Fluorescent immunostainings with Atf4 (A–A″), Bax1 (B–B″), Egr1 (C–C″), IL-18 (D–D″) and Hif1a (E–E″) antibodies show enteric ganglia indicated with dotted lines embedded between the longitudinal- and circular layer of the colon muscularis externa in different experimental setups. All proteins are present at a baseline level in enteric ganglia of control tissues (A–E) and show seemingly more intense staining in DSS-treated mice (A–E′). Enteric neural cells can be recognized by their large euchromatic nuclei visualized by 4′,6-diamidino-2-phenylindole (Dapi) staining. Lm longitudinal layer of muscularis externa, cm circular layer of muscularis externa.

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