Figure 1

Protocol for time-restricted exercise (TRE) implementation. Mice were housed with wireless running wheels available during the early active period (EAP) or late active period (LAP), or remained sedentary (SED), for a 6-week training period (n = 10 mice/group). At the conclusion of the training period, mice were given ad libitum access to the wheel for a 24-h period to determine the effects of time-restricted exercise training on activity rhythms. Hearts were excised to examine the effects of TRE on circadian clock genes and cardiac hypertrophy.