Figure 6 | Scientific Reports

Figure 6

From: In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

Figure 6The alternative text for this image may have been generated using AI.

FRET ratio changes in VSMCs in aorta (a), tenocytes in tail tendon fascicle (b), and isolated VSMCs, in response to macroscopic stretching. Left, snapshots of nuclear fluorescence and the FRET ratio at 0% strain before stretching (left), at the maximum strain (middle), and the final strain during unstretching (right) in one representative experiment. Right, relationships between applied strain and FRET ratio normalized to the value at 0% stretching before stretching. Three specimens were examined in aorta and tendon fascicles and four VSMCs were examined. Unstretching profiles were missed in two of four VSMCs due to technical failures. In (b), an initial FRET profile during stretching is shown with a broken line, as this region corresponds to the straightening of crimped collagen fibers and was not included in evaluation of FRET strain sensitivity. Data were obtained from S2 tension sensors.

Back to article page