Figure 5

EGFR activation regulated inflammation biomarkers in a cell type-dependent manner. The effect of EGF-incubation (E) was compared to control conditions (C) for several parameters. Samples were prepared from cells from multiple donors for each cell type. (a) The concentrations of the pro-inflammatory cytokines IL-8 (number of independent replicates N = 5) and MCP-1 (N = 7) were measured by immunoassay. (b) The expression level of the adhesion molecules VCAM-1 and ICAM-1 were measured by Western-Blot (N = 6). (Representative cut membranes are shown here but whole membranes are displayed in Supplementary Fig. 3). Finally, to estimate the consequences of changes in some of the hereinabove inflammation biomarkers, we performed (c) a leukocyte adhesion assay (N = 5–6) and (d) a permeability assay (N = 7–8). For each experiment performed to evaluate the effect of EGF, the control condition (media with minimal supplementation) was used as reference for each independent replicate. * Wilcoxon test, p < 0.05.