Figure 2
From: Limited high-throughput screening compatibility of the phenuivirus cap-binding domain
Surface plasmon resonance experiments. (a) Crystal structures of SFTSV CBD presented as ribbon diagram with the proteins surface in light grey, the intrinsic cysteines in red and the introduced N-terminal cysteine in blue. The figure was created with PyMOL 2.5. (b) Immobilization of SFTSV CBD Cys via ligand thiol coupling on an amine sensorchip: 1. activation with NHS/EDC, 2. introduction of reactive disulfide groups, 3. injection of SFTSV CBD Cys, 4. deactivation of unreacted disulfide groups. (c) Injection of increasing m7GTP concentrations and (d) of increasing GTP concentrations on the immobilized SFTSV CBD Cys (left side). The right side shows the equilibrium analysis of the data (Analyzer 3 Software, Bruker Daltonics GmbH). Plots were created in GraphPad Prism 9.5.
