Figure 5 | Scientific Reports

Figure 5

From: Reconstitution of C9orf72 GGGGCC repeat-associated non-AUG translation with purified human translation factors

Figure 5

Inhibition of C9-RAN by longer repeat RNA in human PURE-cap. (A, B) Relative expression levels of C9-RAN reporters with varying numbers of repeats in each in vitro translation system. Normalized to 3R-Nluc. (A) The first intron of C9orf72. (B) Mutant intron substituting CUG with AUG codon. The CUG codon located -24 nt upstream of the repeat sequence is predicted as the GA frame start codon32,33,34,35. Error bars represent ± SD from three technical replicates.

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