Figure 3 | Scientific Reports

Figure 3

From: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis

Figure 3

UIR and UUO elicit three transcriptionally distinctive fibroblast clusters. (a) Dot plot of cell type-specific expression of known fibrosis marker genes for manually annotated clusters. Scale: dot size denotes percentage (0, 25, 50, 75, 100) of cells expressing the marker. Color intensity represents average gene expression values. (b) qPCR of fibrosis (Col1a1, Fn1) markers, n = 4–7 per group. **P ≤ 0.01, ****P ≤ 0.0001 compared to control, Student’s t test. (c) Representative IF images of Col1a1 (green) and DAPI (blue) in control, UIR and UUO kidneys. Original magnification, × 40, maximal intensity projection, 0.40 μm/px zoom.

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