Figure 4 | Scientific Reports

Figure 4

From: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis

Figure 4

Three distinctive secretory, contractile and migratory fibroblast clusters emerge in advanced UIR and UUO. (a) Venn diagram displaying unique and shared Fibroblast 1, 2 and 3 marker genes. Complete lists of genes are presented in the Supplementary Table S4. (b–d) GO Biological process of fibroblast clusters unique marker genes vs other populations in control, UIR and UUO, − log2 (P). Representative genes are shown on the left for each biological process. Complete GO analysis is presented in the Supplementary Table S4. (e) Representative images of combined IF for Col1a1 (green), Myh11 (magenta) and DAPI (blue) in control, UIR and UUO kidneys. Original magnification, × 60, maximal intensity projection, 0.06 μm/px zoom. White arrows show Myh11-positive fibroblasts, yellow arrows show Col1a1-positive fibroblasts. (f) GO Biological process of fibroblast clusters 243 shared marker genes, − log2 (P). Complete GO analysis is presented in the Supplementary Table S4.

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