Figure 6 | Scientific Reports

Figure 6

From: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis

Figure 6

Long-term kidney parenchymal remodeling exhibits distal spatial pattern of tubular injury. (a) Dot plot of cell type-specific expression of renal epithelial injury markers for manually annotated clusters in the control, UIR and UUO kidney. Dot size denotes percentage of cells expressing the marker. Color intensity represents average gene expression values. (b) Upper panels—representative images of combined IF for Krt8 (magenta), Umod (yellow), Ecad (white) and DAPI (blue) in control, UIR and UUO kidneys. Original magnification, maximal intensity projection, × 60, 0.14 μm/px zoom. While frames indicate areas of magnification in the lower panels. Lower panels – representative images of combined IF for Krt8 (magenta), Umod (yellow), Ecad (white) and DAPI (blue) in control, UIR and UUO kidneys. Original magnification, × 60, maximal intensity projection, 0.09 μm/px zoom. White arrows indicate the areas of overlap between Krt8 and Umod/Ecad-expressing tubules.

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