Figure 5

Social interaction deficits in the males and females of Shank3^−/− and Cntnap2^−/− mutant mice. (A) Three-chamber sociability test platform of day 1. (B) Three-chamber sociability test platform of day 2. (C) Statistical analysis of time interacting with a stranger mouse (S1). The Shank3^−/− male (N = 17) and female (N = 10) mice spent significantly less time interacting with the stranger mouse than their WT male (N = 24) and female (N = 25) littermates. The data is presented as mean ± SEM. One-way ANOVA followed by Bonferroni- comparison tests were used, F_(3,72) = 8.834. *P < 0.05, **P < 0.01, ns = not significant. (D) Statistical analysis showing time interacting with a novel stranger mouse (S2). The Shank3^−/− male (N = 18) and female (N = 10) mice spent significantly less time interacting with the novel mouse than their WT male (N = 21) and female (N = 27) littermates. The data is presented as mean ± SEM. One-way ANOVA followed by Bonferroni- comparison tests were used, F_(3,72) = 8.834. *P < 0.05, ns = not significant. (E) Statistical analysis of time interacting with S1. The Cntnap2^−/− male (N = 26) and female (N = 23) mice spent significantly less time interacting with the stranger mouse than their WT male (N = 18) and female (N = 16) counterparts. The data is presented as mean ± SEM. One-way ANOVA followed by Bonferroni- comparison tests were used, F_(3,79) = 5.033. *P < 0.05, **P < 0.01, ns = not significant. (F) Statistical analysis of time interacting with S2. The Cntnap2^−/− male (N = 23) and female (N = 19) mice spent significantly less time interacting with S2 than the WT male (N = 16) and female (N = 16) mice. The data is presented as mean ± SEM. One-way ANOVA followed by Bonferroni- comparison tests were used, F_(3,70) = 8.685. **P < 0.01, ns = not significant.