Table 1 Results of the histopathological analysis, ZN stain, ELISA, PCR, and bacteriological culture from the 14 animals included in the study.

From: MicroRNAs modulate immunological and inflammatory responses in Holstein cattle naturally infected with Mycobacterium avium subsp. paratuberculosis

Animal ID

Histopathological analysis

ELISA

Fecal PCR

Fecal culture

Tissue PCR

Tissue culture

Microscopic

Macroscopic

ZN

1

Negative

No

Negative

Negative

Negative

Negative

Negative

Negative

2

Negative

No

Negative

Negative

Negative

Negative

Negative

Negative

3

Negative

No

Negative

Negative

Negative

Negative

Negative

Negative

15

Negative

No

Negative

Negative

Negative

Negative

Negative

Negative

4

Focal

No

Negative

Negative

Negative

Negative

Positive

Negative

5

Focal

No

Negative

Negative

Negative

Negative

Positive

Low

6

Focal

No

Negative

Negative

Negative

Negative

Positive

Negative

7

Focal

No

Negative

Negative

Negative

Negative

Positive

Negative

8

Focal

No

Negative

Positive

Positive

Negative

Positive

Medium

10

Diffuse

Yes

Positive

Positive

Positive

Negative

Positive

Heavy

11

Diffuse

Yes

Positive

Positive

Positive

Negative

Positive

Heavy

12

Diffuse

Yes

Positive

Positive

Positive

Heavy

Positive

Heavy

13

Diffuse

Yes

Positive

Positive

Positive

Negative

Positive

Low

14

Diffuse

Yes

Positive

Positive

Positive

Negative

Positive

Heavy

  1. Bacterial load was classified as low (< 10 CFU), medium (between 10 and 50 CFU) or heavy (> 50 CFU).
  2. N Ziehl–Neelsen, CFU colony forming units.