Figure 6 | Scientific Reports

Figure 6

From: Embryonic-stem-cell-derived mesenchymal stem cells relieve experimental contact urticaria by regulating the functions of mast cells and T cells

Figure 6

TGF-β gene expression induced by M-MSCs. (A,B) M-MSCs were co-cultured with splenocytes or BMMCs for 24 h. The levels of human Hgf, Ido1, Pdl1, Il10, Pge2, and Tgfb in the M-MSCs were examined by RT-PCR. Gapdh was used as the control. Original gels are presented in Supplementary Fig. S2. Significant differences against the M-MSCs, *p < 0.05; n.s., not significant by Student’s t test. (n = 5). (C,D) M-MSCs or BM-MSCs were co-cultured with splenic T cells or BMMCs for 24 h. The human Hgf, Ido1, Pdl1, Il10, Pge2, and Tgfb mRNA were measured using RT-PCR. Original gels are presented in Supplementary Figs. S3 and S4. Representative images and graphs are shown as mean ± SEM from five independent experiments. Significant differences against the M-MSCs or BM-MSCs, *p < 0.05, n.s., not significant by Student’s t test (n = 5).

Back to article page