Figure 2
From: Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice
Atp10A deletion does not result in changes to glucose homeostasis after 12 weeks of HFD. (a) Male and (b) female mice were gavaged with 20% dextrose (time 0 on the graph) after a 5-h fast then an oral glucose tolerance test (OGTT) was performed. The inset shows the area under the curve (AUC) of the blood glucose (mg/dl) during the OGTT, normalized to fasting blood glucose (points before time 0 on the graph), ***P = 0.0003. Male: 10A+/+ n = 6,10A−/− n = 6; Female: 10A+/+ n = 12, 10A−/− n = 6) (c) Fasting blood glucose was measured from tail blood after a 5 h fast, via a glucometer (Male: 10A+/+ n = 10, 10A−/− n = 11; Female: 10A+/+ n = 19, 10A−/− n = 14). (d) Fasting plasma insulin was measured after a 5 h fast (Male: 10A+/+ n = 8, 10A−/− n = 10; Female: 10A+/+ n = 13,10A−/− n = 9). Insulin was measured from serum collected during the OGTT shown in Fig. 2a,b at the (e) 30-min time point (T30) and the (f) 60-min time point (T60), (Male: E: 10A+/+ n = 4, 10A−/− n = 4, F: 10A+/+ n = 6, 10A−/− n = 5; Female: 10A+/+ n = 8, 10A−/− n = 6). P value by (a,b, not AUC) 2-way ANOVA with Sidak’s multiple comparison or (a,b (AUC), c–f) unpaired t-tests.
