Figure 5
From: Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice
Atp10A deletion causes changes to liver lipid metabolism in female mice after 12 weeks of HFD. (a) FFAs were measured from flash frozen livers via gas chromatography. Livers were collected after a 5 h fast or after a 5 h fast followed by an OGTT. (FFA: 10A+/+ n = 15,10A−/− n = 7, *P = 0.0201). The saturation of liver (b) TG, PL, and (c) CE species was determined by gas chromatography. The fold change of monounsaturated vs saturated TG, PL, CE is shown, TG: **P = 0.0025, ***P = 0.0001, *P = 0.0443, PL: *P = 0.0297, *P = 0.0281 (10A+/+ n = 16, 10A−/− n = 7). CE: *P = 0.0223 (10A+/+ n = 6, 10A−/− n = 6). (d) Representative images of livers stained with H + E (5x) or Oil Red O (63x). The coalescence of the Oil Red O stain is indicated by yellow asterisks (artifact) and the arrows point to neutral lipids stained by Oil Red O. Scale bars = 10 μM. (e) Liver sections were scored using the Oil Red O Score described in the Materials and Methods, *P = 0.0327 (10A+/+ n = 6, 10A−/− n = 10). (f) The diameters of Oil Red O positive lipid droplets (LD) in the liver sections with a score of 2 were measured using ImageJ. The red line in the graph represents the SEM, ****P = < 0.0001 (10A+/+,120 LDs measured, n = 2, 10A−/−, 203 LDs measured, n = 3). P value by (a–c, f) unpaired t-test or (e) Mann–Whitney U test.
