Figure 5
From: Tuning of liver circadian transcriptome rhythms by thyroid hormone state in male mice
Identification of thyroid hormone-responsive genes by mesor comparison. (A) Heatmap shows the 516 TH-dose responsive genes with differential mesor expression among each group. (B) Gene set enrichment analysis (GSEA) of mesor-altered genes is shown for each condition. (C) Genes pertaining to similar biological pathways identified in B were normalized by CON mesor and plotted. FA biosynthesis pathway comprises lipid metabolic process, unsaturated fatty acid biosynthetic process, long-chain fatty-acyl-CoA biosynthetic process, diacylglycerol biosynthetic process, negative regulation of fatty acid biosynthetic process, and lipid storage. FA catabolism pathway is comprised of acyl-CoA metabolic process, lipid metabolic process, fatty acid metabolic process, very-low-density lipoprotein particle assembly processes. Cholesterol and bile acid metabolism pathways comprise cholesterol homeostasis, cholesterol metabolic process, steroid metabolism, and bile acid signaling pathway. Carbohydrate metabolism pathways are comprised of carbohydrate metabolic process, glycogen metabolic process, ATP metabolic process, and glucose homeostasis. (D) Selected biomarkers for TH state at all time points (additional genes can be found in Supplementary file 5). Presence (R) or absence (NR) of circadian rhythm by CircaCompare (p value < 0.05) is depicted. n = 3–4 for all ZTs and groups.
