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Figure 1

From: Native PLGA nanoparticles attenuate Aβ-seed induced tau aggregation under in vitro conditions: potential implication in Alzheimer’s disease pathology

Figure 1

Tau aggregation in the presence and absence of heparin. (A–C) ThT kinetic assays showing the spontaneous aggregation curves (A) and the corresponding fluorescence (B) as well as STEM (C) images of 0N4R tau in the absence and presence of heparin over 40 h incubation. Note the aggregation of 0N4R tau increases dose-dependently in presence of heparin which reaches plateaus over time as indicated by ThT fluorescence levels. Tau, as expected, did not aggregate in the absence of heparin (A). All ThT kinetic graphs represented average mean ± SEM of three separate experiments, each performed with six replicates for each condition. The fluorescence (B) and STEM (C) images reflecting tau aggregation at different concentrations were taken following 40 h incubation. (D,E) DLS analysis showing differential increase in the diameter of aggregated tau as a function of concentrations in the presence and absence of heparin following 40 h incubation as depicted by size distribution curves (D) and histogram (E) representing average aggregate size (left side Y axis) and mean peaks of tau aggregates for a given condition (right side Y axis).

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