Figure 3
From: Low dose cadmium exposure regulates miR-381–ANO1 interaction in airway epithelial cells
miR-381 negatively regulates ANO1 expression in Cd exposed CBECs. (a) miRNAs regulating ANO1 mRNA sequence were analyzed from different miRNA databases—TargetScan, miRDB and PicTAR.Venn diagram using BioVenn platform. (b) Putative binding sequence of miR-381 in wild type 3ʹ-UTR of ANO1. (c) Relative expression of miR-381 and ANO1 in presence of miR-381 inhibitor (miR-381 IN) or mimic (miR-381 MM). Expression analysis was performed by drawing comparisons against miR-381 negative control (miR-NC). (d) Expression of ANO1 upon miR-381 overexpression and inhibition in CBECs represented with fluorescence microscopy at × 20 magnification.
