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Figure 2

From: SIRT1 as a potential key regulator for mediating apoptosis in oropharyngeal cancer using cyclophosphamide and all-trans retinoic acid

Figure 2Figure 2Figure 2

Effect of cyclophosphamide (C) (26.5 μM), Retinoic acid (R) (40 μM) and their combination on apoptosis parameters. Flow cytometry scatterplots for (a) control, (b) cyclophosphamide, (c) retinoic acid, (d) their combination, (e) quantitative analysis of the total apoptosis rate and (f) quantitative analysis of Necrosis rate. Real time mRNA expression of (g) Bax, (h) Bcl-2, (i) Bcl-xl, (j) PAR-4, in addition to (k, l) caspase 3 and 9 levels, (m, n) Bax and Bcl-2 levels using ELISA technique. Results were expressed as means ± SD of two independent experiments performed in duplicates (n = 4). Statistical significance of results was analyzed using one-way ANOVA followed by Tukey’s multiple comparison test (a) Significantly different from the control group (b) Significantly different from Cyclophosphamide treated group, (c) Significantly different from Retinoic acid treated group at P ˂ 0.05.

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